CD22 antibody

Catalog No. ABIN370868

The Rat Monoclonal anti-CD22 antibody has been validated for FACS and Func. It is suitable to detect CD22 in samples from Mouse.

Quick Overview for CD22 antibody (ABIN370868)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: 2D6

Product Details anti-CD22 Antibody

Specificity: This antibody is specific to CD22 (Mr 150 kDa), a B Lymphocyte Adhesion Molecule.

Characteristics: Synonyms: SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2,B-lymphocyte cell adhesion molecule

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. (Ref.2)Functional Assay: Blocking of B cell homotypic adhesion. (Ref.2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. (Ref.1,2) CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. (Ref.2-4) Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation. (Ref.6-9)Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2

Gene ID: 12483

UniProt: P35329